Detection of IgG and IgE antibodies to Aspergillus fumigatus in human sera by immunogold assay |
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| Authors: | Harish C Gugnani Kari E Reijula Viswanath P Kurup Jordan N Fink |
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| Institution: | (1) Research Service, Clement J. Zablocki Department of Veterans Affairs Medical Center, and the Allergy-Immunology Division, Department of Medicine, Medical College of Wisconsin, USA;(2) Present address: Department of Microbiology, University of Nigeria, Nsukka, Nigeria;(3) Present address: Oulu Regional Institute of Occupational Health, Oulu, Finland |
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| Abstract: | An immunogold assay (IGA) was developed to detect IgG and IgE antibodies to Aspergillus fumigatus. Sixteen sera from patients with allergic bronchopulmonary aspergillosis (ABPA), aspergilloma, and normal controls were studied. All sera were also evaluated for antibodies against A. fumigatus by biotin-avidin linked enzyme immunosorbent assay (BALISA) and by agar gel double diffusion method. A. fumigatus specific IgG and IgE antibodies could be detected by IGA in all the patients' sera but not in the sera of normal controls. Both IgG and IgE antibodies to A. fumigatus could be demonstrated in all the sera by BALISA and normal controls showed only low levels of these antibodies. There was a positive correlation between the degree of reactivity detected by IGA, the BALISA titer and the precipitins by agar gel diffusion. It can be concluded that IGA is a reliable, sensitive and simple method capable of detecting both IgG and IgE antibodies against A. fumigatus in patient serum. |
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| Keywords: | Aspergillus immunogold staining biotin-avidin linked immunosorbent assay double immunodiffusion aspergillosis aspergilloma |
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