Expression of an agrocin-encoding plasmid of Agrobacterium tumefaciens in Rhizobium meliloti |
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| Authors: | Mavis Hendson Jennifer A Thomson |
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| Institution: | Department of Genetics, University of the Witwatersrand, 1 Jan Smuts Avenue, Johannesburg 2001, South Africa |
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| Abstract: | Plasmid RP4 was used to mobilize the agrocin 84-encoding plasmid, pAg396, from Agrobacterium tumefaciens strain 396 to A. tumefaciens C58 and C58CI as well as Rhizobium meliloti. It was transferred to, but not stably maintained in, R. leguminosarum. It could not be transferred to R. lupini, R. japonicum or R. trifolii. Plasmid pAg396 did not segregate in R. meliloti and produced levels of agrocin comparable to the parental strain A. tumefaciens 396. The potential of agrocin producing R. meliloti in biological control of crown gall is being investigated. |
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