Carbon catabolite repression of invertase during batch cultivations of Saccharomyces cerevisiae: the role of glucose, fructose, and mannose |
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Authors: | J Dynesen H P Smits L Olsson J Nielsen |
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Institution: | (1) Department of Biotechnology, Center for Process Biotechnology, Technical University of Denmark, DK-2800 Lyngby, Denmark e-mail: lo@ibt.dtu.dk Fax: +45-45-88-41-48, DK |
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Abstract: | When Saccharomyces cerevisiae are grown on a mixture of glucose and another fermentable sugar such as sucrose, maltose or galactose, the metabolism is
diauxic, i.e. glucose is metabolized first, whereas the other sugars are metabolized when glucose is exhausted. This phenomenon
is a consequence of glucose repression, or more generally, catabolite repression. Besides glucose, the hexoses fructose and
mannose are generally also believed to trigger catabolite repression. In this study, batch fermentations of S. cerevisiae in mixtures of sucrose and either glucose, fructose or mannose were performed. It was found that the utilization of sucrose
is inhibited by concentrations of either glucose or fructose higher than 5 g/l, and thus that glucose and fructose are equally
capable of exerting catabolite repression. However, sucrose was found to be hydrolyzed to glucose and fructose, even when
the mannose concentration was as high as 17 g/l, indicating, that mannose is not a repressing sugar. It is suggested that
the capability to trigger catabolite repression is connected to hexokinase PII, which is involved in the in vivo phosphorylation
of glucose and fructose.
Received: 5 May 1998 / Received revision: 3 August 1998 / Accepted: 8 August 1998 |
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