Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit |
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| Authors: | T Barkas J M Gabriel A Mauron G J Hughes B Roth C Alliod S J Tzartos M Ballivet |
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| Affiliation: | Service of Neurology, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland. |
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| Abstract: | Monoclonal antibodies (mAbs) to the main immunogenic region (MIR) bind to fusion proteins containing region 37-200 of the alpha chain of Torpedo, mouse, and chicken nicotinic acetylcholine receptor. In the case of the mouse alpha chain, these mAbs react with sequence 61-216 but not with 74-216. A synthetic peptide M1, containing residues 61-76 of the mouse alpha chain, also binds these anti-MIR mAbs, showing that all or part of their binding site is included in this region. The conformational dependence and epitope specificity of the mAbs are discussed. |
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