Failure of zinc to inhibit hyperthermia-induced apoptosis predicts differential nuclease activity detection by comet assay |
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Authors: | D W Fairbairn K L O'Neill |
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Institution: | (1) Department of Microbiology, Brigham Young University, Provo, Utah, USA;(2) Department of Microbiology, BYU, 751 WIDB, 84602 Provo, Utah, USA |
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Abstract: | Apoptotic DNA cleavage generally proceeds in two stages, first producing large 50–300kb fragments, and later oligonucleosomal pieces which create the characteristic DNA ladder. We show that zinc treatment of hyperthermia-induced apoptotic cultures is sufficient to prevent ladder formation, but not apoptosis (all features of which were inhibited by actinomycin D and cycloheximide). DNA damage measured in single cells using the comet (single cell gel) assay is detectable in zinc treated cultures using both alkaline and non-denaturing conditions. Both assays predict the same fraction of cells undergoing apoptosis, and damage is detectable earlier than shown by DNA ladder appearance. We conclude that the comet assay is detecting damage consistent with the initial 50–300kb fragments. Additionally, various cell lines when heattreated follow different temporal pathways or display differential apoptotic phenotypes. Also, we were unable to demonstrate an apoptotic window for cells refractory to hyperthermia by increasing the heat load. |
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Keywords: | Apoptosis comet assay hyperthermia zinc |
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