Transformation with a mutant Arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides |
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Authors: | George W. Haughn Julie Smith Barbara Mazur Chris Somerville |
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Affiliation: | (1) MSU-DOE Plant Research Laboratory, Michigan State University, 48824 East Lansing, MI, USA;(2) Central Research and Development Department, Du Pont Experimental Station 402, 19898 Wilmington, DE, USA |
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Abstract: | Summary A gene encoding acetolactate synthase was cloned from a chlorsulfuron-resistant mutant of Arabidopsis. The DNA sequence of the mutant gene differed from that of the wild type by a single base pair substitution. When introduced into tobacco by Ti plasmid-mediated transformation the gene conferred a high level of herbicide resistance. These results suggest that the cloned gene may confer agronomically useful levels of herbicide resistnace in other crop species, and that it may be useful as a selectable marker for plant transformation experiments. |
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Keywords: | Ti plasmid Chlorsulfuron Transformation Acetohydroxy acid synthase |
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