| 亚适宜温光环境下不同硝铵比营养液对黄瓜幼苗生长、氮吸收和代谢的影响 |
| |
| 引用本文： | 张小翠,刘玉梅,白龙强,贺超兴,于贤昌,李衍素.亚适宜温光环境下不同硝铵比营养液对黄瓜幼苗生长、氮吸收和代谢的影响[J].生态学杂志,2016,27(8):2527-2534. |
| |
| 作者姓名： | 张小翠 刘玉梅 白龙强 贺超兴 于贤昌 李衍素 |
| |
| 作者单位： | 1.中国农业科学院蔬菜花卉研究所, 北京 100081;;2.菏泽学院园林工程系, 山东菏泽 274000 |
| |
| 基金项目： | 本文由国家自然科学基金项目(31101583,31272212)、现代农业产业技术体系建设专项(CARS-25-C-01)、中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)、公益性行业(农业)科研专项(201203005)和农业部园艺作物生物学与种质创制重点实验室项目资助 |
| |
| 摘 要： | 以‘中农26’黄瓜为试材,研究亚适宜温光(18 ℃/10 ℃,180±20 μmol·m-2·s-1)下不同硝铵比(26:2、21:7和14:14)营养液对黄瓜幼苗生长和生理特性的影响.结果表明:硝铵比为21:7处理的黄瓜总根长最长、根体积和根表面积最大、根尖数最多;根中H+-ATPase活性最高;硝态氮转运蛋白(NRT)和铵转运蛋白(AMT)基因表达量也升高,提高了根系的氮吸收能力;叶中硝酸还原酶(NR)、谷氨酰胺合成酶(GS)和谷氨酸合酶(GOGAT)活性较高,提高了体内氮代谢能力,使黄瓜氮含量和干物质积累显著增加.亚适宜温光下,营养液中硝铵比为21:7时,黄瓜幼苗干质量分别比26:2和14:14处理增加14.0%和19.3%.亚适宜温光下,可通过调整营养液中硝铵比,提高黄瓜氮吸收与代谢能力,缓解亚适宜温光对黄瓜幼苗的不利影响,促进黄瓜生长.
|
| 关 键 词： | 黄瓜 硝铵比 氮吸收 氮代谢 |
| 收稿时间： | 2016-01-12 |
Effects of different NO3--N/NH4+-N ratios on cucumber seedlings growth,nitrogen absorption and metabolism under suboptimal temperature and light intensity |
| |
| ZHANG Xiao-cui,LIU Yu-mei,BAI Long-qiang,HE Chao-xing,YU Xian-chang,LI Yan-su.Effects of different NO3--N/NH4+-N ratios on cucumber seedlings growth,nitrogen absorption and metabolism under suboptimal temperature and light intensity[J].Chinese Journal of Ecology,2016,27(8):2527-2534. |
| |
| Authors: | ZHANG Xiao-cui LIU Yu-mei BAI Long-qiang HE Chao-xing YU Xian-chang LI Yan-su |
| |
| Institution: | 1.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;;2.Department of Landscape Engineering, Heze University, Heze 274000, Shandong, China |
| |
| Abstract: | Cucumber (cv. Zhongnong 26) was used as material, the effects of NO3--N/NH4+-N ratios on growth and physiological characteristics of cucumber seedlings under suboptimal temperature and light intensity (18 ℃/10 ℃,180 ± 20 μmol·m-2·s-1) were studied. Total nitrogen in the nutrient solution was equal and three NO3--N/NH4+-N ratios, 26:2, 21:7 and 14:14, were applied as treatments. The results showed that cucumber treated by NO3--N/NH4+-N=21:7 had the longest total root length, the biggest root volume and root surface area, and the maximum number of root tips. H+-ATPase activity and relative expression of genes encoding nitrate transporter (NRT) and ammonium transporter (AMT) in cucumber roots were increased significantly by the treatment of NO3--N/NH4+-N=21:7. In addition, nitrate reductase (NR), glutamine synthetase (GS) and glutamate synthase (GOGAT) in cucumber leaves under the treatment of NO3--N/NH4+-N=21:7 were higher. As a result, the nitrogen content and biomass of cucumber were significantly increased. Compared with the plants under the treatment of NO3--N/NH4+-N=26:2 or 14:14, cucumber seedlings under the treatment of NO3--N/NH4+-N=21:7 had the highest biomass and total dry mass (DM) which were increased by 14.0% and 19.3% respectively under suboptimal temperature and light intensity. In conclusion, under suboptimal environmental conditions, NO3- -N/NH4+-N ratio could be adjusted to increase nitrogen absorption and metabolism of cucumber and alleviate the de-trimental effects caused by suboptimal conditions and promoted the cucumber growth. |
| |
| Keywords: | cucumber NO3--N/NH4+-N ratio nitrogen absorption nitrogen metabolism |
|
| 点击此处可从《生态学杂志》浏览原始摘要信息 |
| 点击此处可从《生态学杂志》下载免费的PDF全文 |
|
