| 有机物料腐熟剂中纤维素酶活力测定方法的验证 |
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| 引用本文： | 吴红艳,王智学,陈飞,郭玲玲,修翠娟. 有机物料腐熟剂中纤维素酶活力测定方法的验证[J]. 微生物学杂志, 2011, 31(4): 107-109 |
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| 作者姓名： | 吴红艳 王智学 陈飞 郭玲玲 修翠娟 |
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| 作者单位： | 辽宁省微生物科学研究院,辽宁朝阳,122000 |
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| 基金项目： | 国家科技试验与示范(国科发农(2010)297号); 辽宁省科技攻关项目(2009301005) |
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| 摘 要： | 验证有机物料腐熟剂中纤维素酶活力测定方法,为本品活力测定方法的确定提供科学依据。通过DNS显色,对测定方法进行线性关系、准确度（加样回收率）、精密度、重复性等进行验证。此方法线性关系良好,R=0.999 0,平均加样回收率为98.29%,RSD%=0.513 3%（n=9）小于2%。精密度和重复性均符合要求。本测定方法稳定、可靠,可以作为有机物料腐熟剂中纤维素酶活力的测定方法。
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| 关 键 词： | 有机物料腐熟剂 纤维素酶 方法验证 |
The Test Method of Cellulase Activity of Organic Material Full-Decomposing Agent |
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| WU Hong-yan,WANG Zhi-xue,CHEN Fei,GUO Ling-ling,XIU Cui-juan. The Test Method of Cellulase Activity of Organic Material Full-Decomposing Agent[J]. Journal of Microbiology, 2011, 31(4): 107-109 |
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| Authors: | WU Hong-yan WANG Zhi-xue CHEN Fei GUO Ling-ling XIU Cui-juan |
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| Affiliation: | WU Hong-yan,WANG Zhi-xue,CHEN Fei,GUO Ling-ling,XIU Cui-juan(Liaoning Res.Acad.of Microbiol.Sci.,Chaoyang 122000) |
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| Abstract: | The test method of cellulase activity of organic material full-decomposing agent(OMFDA) was verified in order to provide a scientific basis,the methods were determined with its linear relationship,accuracy(recovery of adding sample),precision and repeatability by DNS developing.The results showed that the linear relationship of the method was good,R=0.999 0,with the average recovery of adding sample at 98.29%,RSD% =0.513 3%(n=9) less than 2%.And the precision and repeatability met the requirements.Therefore... |
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| Keywords: | organic material full-decomposing agent(OMFDA) cellulase method verification |
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