Phosphinothricin stimulates somatic embryogenesis in grape (Vitis sp. L.) |
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| Authors: | Dominique Hébert-Soulé Julie R. Kikkert Bruce I. Reisch |
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| Affiliation: | (1) Institut National de la Recherche Agronomique, Station de Génétique et d'Amélioration des Plantes de Montp ellier, Domaine de Melgueil, F-34130 Mauguio, France;(2) Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, 14456 Geneva, NY, USA;(3) Present address: Residence les Fleurs, 63 Chemin de Pelleport, 31500 Toulouse, France |
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| Abstract: | The effect of phosphinothricin concentration on embryo production from an embryogenic callus of  Chancellor (Vitis L. complex interspecific hybrid) was tested. Embryogenic callus was cultured on medium supplemented with nine phosphinothricin concentrations (0, 0.1, 0.5, 1, 1.5, 2, 3, 5, and 10 mg/l). The highest number of embryos per plate was observed at 0.5 mg/l phosphinothricin. The use of phosphinothricin to stimulate embryo production did not affect embryo germination and plantlet formation. Three germination techniques were compared. Embryo dehydration or growth on Transfergelsolidified medium gave higher germination rates than chilling treatments. Most germinated somatic embryos produced secondary embryos from the hypocotyl after a few weeks of culture. Regardless of the germination technique, the plantlet conversion rate was very low.Abbreviations AC activated charcoal - 2,4-D 2,4-dichlorophenoxyacetic acid - BA 6-benzylaminopurine - MS Murashige and Skoog (1962) basal medium - NN Nitsch and Nitsch (1969) medium - PPT phosphinothricin |
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