| 乙醛脱氢酶2基因在毕赤酵母中的表达和分离纯化 |
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| 引用本文： | 陈瑞,方剑,王静涵. 乙醛脱氢酶2基因在毕赤酵母中的表达和分离纯化[J]. 生物加工过程, 2013, 0(6): 34-37 |
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| 作者姓名： | 陈瑞 方剑 王静涵 |
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| 作者单位： | [1]皖西学院生物与制药工程学院,六安237012 [2]皖西学院安徽省植物生物技术实验实训中心,六安237012 |
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| 基金项目： | 大学生创新创业计划项目（201210376007）; 六安市定向委托皖西学院市级研究项目（2012LW001） |
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| 摘 要： | 将乙醛脱氢酶2（ALDH2）基因整合到质粒pPIC9K上,构建重组表达载体pPIC9K-coALDH2,用电转导将表达质粒pPIC9K-coALDH2转化至毕赤酵母GS115中,在毕赤酵母中表达经密码子改造的ALDH2。结果表明：重组基因工程菌GS115（pPIC9K-coALDH2）发酵液中蛋白质量浓度为8.40 mg/L,1 mL发酵液中酶活为11.35 mU。
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| 关 键 词： | 乙醛脱氢酶 GS115 表达 |
Expression of acetaldehyde dehydrogenase 2 gene in Pichia pastoris |
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| CHEN Rui,FANG Jian,WANG Jinghan. Expression of acetaldehyde dehydrogenase 2 gene in Pichia pastoris[J]. Chinese Journal of Bioprocess Engineering, 2013, 0(6): 34-37 |
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| Authors: | CHEN Rui FANG Jian WANG Jinghan |
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| Affiliation: | 1. College of Biology and Pharmaceutical Engineering, West Anhui University, Lu＇an 237012, China ; 2. Experiment and Training Center of Plant Biotechnology of Anhui Province, West Anhui University, Lu＇an 237012, China) |
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| Abstract: | To obtain acetaldehyde dehydrogenase 2 （ALDH2）, the pPIC9K-ALDH2 was linearized and transformed by electroporation into Pichia pastoris GSll5, and the new engineering bacteria GSll5 （pPIC9K-colALDH） for overexpressing ALDH2 was obtained. The results showed that the content of target protein was 8.40 mg./L and the enzymatic activities in culture recombinant Pichia strains reached 11.35 mU/mL, and it was higher than that in extracellular expression. |
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| Keywords: | acetaldehyde dehydrogenase GS115 express |
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