| 硫胺素对Arthrobacter sp.A302环磷酸腺苷发酵的影响 |
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| 引用本文： | 要世伟,牛欢青,陈勇,应汉杰.硫胺素对Arthrobacter sp.A302环磷酸腺苷发酵的影响[J].生物加工过程,2013(6):19-23. |
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| 作者姓名： | 要世伟 牛欢青 陈勇 应汉杰 |
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| 作者单位： | 南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京210009 |
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| 基金项目： | 国家杰出青年基金（21025625）; 国家高技术研究发展计划（863计划）（2012AA021203）; 国家重点基础研究发展计划（973计划）（2011CBA00806）; 国家自然科学基金青年基金（21106070）; 教育部长江学者和创新发展计划（PCSIRT）; 江苏省自然科学基金（SBK201150207）; 江苏省高校优势学科建设工程项目（PAPD） |
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| 摘 要： | 研究在培养基中加入硫胺素（V B1）对cAMP发酵的影响。结果表明：V B1的最适添加量为0.5 g/L,与对照组相比,环磷酸腺苷（cAMP）产量和细胞干质量分别提高了36.4%和41.8%,达到7.5和7.8 g/L。琥珀酸和α-酮戊二酸的含量有明显的提高,平均提高了43.59%和40.77%;同时,主要副产物乙酸的含量没有明显变化。在发酵过程中,V B1的加入对ATP的含量及与cAMP合成密切相关的几种酶的活性也有显著的影响。
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| 关 键 词： | 硫胺素（VB1） cAMP Arthrobacter |
Effects of thiamin on production of cyclic adenosine monophosphate by Arthrobacter sp. A302 |
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| YAO Shiwei,NIU Huanqing,CHEN Yong,YING Hanjie.Effects of thiamin on production of cyclic adenosine monophosphate by Arthrobacter sp. A302[J].Chinese Journal of Bioprocess Engineering,2013(6):19-23. |
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| Authors: | YAO Shiwei NIU Huanqing CHEN Yong YING Hanjie |
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| Institution: | ( State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China) |
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| Abstract: | The effects of addition of thiamine （VB1） on cyclic adenosine monophosphate （cAMP） production by Arthrobacter sp. A302 were investigated. The results showed that the addition of 0.5 g/L VB1 was optimum for cAMP production and cell growth. The cAMP yield and the biomass reached 7.5 g/L and 7.8 g/L, and they were improved by 36.4% and 41.8% , respectively, compared with the control. The concentrations of succinic acid and a-ketoglutarate increased by 43.59% and 40.77%, and the production of acetic acid had no obvious change by addition 0.5 g/L VB1. The addition of VB1 also affected the content of intracellular ATP and activities of several enzymes closely related to biosynthesis of cAMP. |
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| Keywords: | Thiamine （ VB1 ） cAMP Arthrobacter |
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