Neuroprotective and Antioxidative Effect of Cactus Polysaccharides In Vivo and In Vitro |
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Authors: | Xianju Huang Qin Li Huige Li Lianjun Guo |
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Institution: | (1) Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13#, 430030 Wuhan, People’s Republic of China;(2) College of Pharmacy, South-Central University for Nationalities, 430074 Wuhan, People’s Republic of China;(3) Department of Pharmacology, Johannes Gutenberg University, Mainz, Germany |
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Abstract: | Cactus polysaccharides (CP), some of the active components in Opuntia dillenii Haw have been reported to display neuroprotective effects in rat brain slices. In the present study, we investigated the
neuroprotective properties of CP and their potential mechanisms on brain ischemia-reperfusion injury in rats, and on oxidative
stress-induced damage in PC12 cells. Male Sprague–Dawley rats with ischemia following middle cerebral artery occlusion and
reperfusion were investigated. CP (200 mg/kg) significantly decreased the neurological deficit score, reduced infarct volume,
decreased neuronal loss in cerebral cortex, and remarkably reduced the protein synthesis of inducible nitric oxide synthase
which were induced by ischemia and reperfusion. Otherwise, the protective effect of CP was confirmed in in vitro study. CP
protected PC12 cells against hydrogen peroxide (H2O2) insult. Pretreatment with CP prior to H2O2 exposure significantly elevated cell viability, reduced H2O2-induced apoptosis, and decreased both intracellular and total accumulation of reactive oxygen species (ROS) production. Furthermore,
CP also reversed the upregulation of Bax/Bcl-2 mRNA ratio, the downstream cascade following ROS. These results suggest that
CP may be a candidate compound for the treatment of ischemia and oxidative stress-induced neurodegenerative disease. |
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