Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.) |
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Authors: | M J Hayden P Stephenson A M Logojan D Khatkar C Rogers J Elsden R M D Koebner J W Snape P J Sharp |
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Institution: | (1) Plant Functional Genomics Center, University of Adelaide, Hartley Grove, Urrbrae, SA, 5064, Australia;(2) Molecular Plant Breeding CRC, PMB 1, Glen Osmond, SA, 5064, Australia;(3) Crop Genetics Department, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK;(4) Plant Breeding Institute, University of Sydney, PMB11, Camden, NSW, 2570, Australia;(5) Value Added Wheat CRC, LB1345, North Ryde, NSW, 2113, Australia |
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Abstract: | The density of SSRs on the published genetic map of bread wheat (Triticum aestivum L.) has steadily increased over the last few years. This has improved the efficiency of marker-assisted breeding and certain types of genetic research by providing more choice in the quality of SSRs and a greater chance of finding polymorphic markers in any cross for a chromosomal region of interest. Increased SSR density on the published wheat genetic map will further enhance breeding and research efforts. Here, sequence-tagged microsatellite profiling (STMP) is demonstrated as a rapid technique for the economical development of anonymous genomic SSRs to increase marker density on the wheat genetic map. A total of 684 polymorphic sequence-tagged microsatellites (STMs) were developed, and 380 were genetically mapped in three mapping populations, with 296 being mapped in the International Triticeae Mapping Initiative W7984 × Opata85 recombinant inbred cross. Across the three populations, a total of 479 STM loci were mapped. Several technological advantages of STMs over conventional SSRs were also observed. These include reduced marker deployment costs for fluorescent-based SSR analysis, and increased genotyping throughput by more efficient electrophoretic separation of STMs and a high amenability to multiplex PCR.Electronic Supplementary Material Supplementary material is available in the online version of this article at and is accessible for authorized users. |
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