Bioluminescence intensity difference observed in luminous bacteria groups with different motility |
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Authors: | S Sasaki T Okamoto T Fujii |
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Institution: | School of Bionics, Tokyo University of Technology, Tokyo, Japan; Institute of Industrial science, The University of Tokyo, Tokyo, Japan |
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Abstract: | Aims: The aim of this study was to compare the luminescent intensity of bioluminescence from marine luminous bacteria with different motility. Methods and Results: Luminescent bacteria were separated according to their motility using a microfluidic device. The cell densities of the separated samples were measured using a counting plate. The luminescent intensity of the separated samples was measured using a luminometer. The luminescent intensity per cell was calculated, and the values from the mobile (swimmers) and the nonmobile cells (nonswimmers) per single cell were compared; as a result, the former were proved to be larger than the latter. Conclusions: Microfluidics were shown to be effective for the separation of bioluminescent bacteria and the bioluminescent intensity difference per cell was recognized with this experiment. Significance and Impact of the Study: This study introduced for the first time a method to examine the individual cell function of Photobacterium kishitanii . |
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Keywords: | bacteria luminescence microfluidics motility photobacterium |
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