A composite map of expressed sequences and phenotypic traits of the sunflower (Helianthus annuus L.) genome |
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Authors: | L Gentzbittel E Mestries S Mouzeyar F Mazeyrat S Badaoui F Vear D Tourvieille de Labrouhe P Nicolas |
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Institution: | (1) Gie Cartisol, 20 rue Bachaumont, 75002 Paris, France, FR;(2) Groupe de Recherche Auvergne Tournesol-INRA, Station d’Amélioration des Plantes et de Pathologie végétale, Domaine de Crouelle, 234 avenue du Brézet, 63000 Clermont-Ferrand, France-UA UBP-INRA OVGV, Campus des Cézeaux, 24 Avenue des Landais, 63177 Aubiere, France, FR |
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Abstract: | A map of the sunflower genome, based on expressed sequences and consisting of 273 loci, was constructed. The map incorporates
data from seven F2 populations, for a total of 1115 individuals. Two hundred and fourty five loci corresponding to 170 anonymous cDNA markers
and four loci for morphological markers were mapped. We also mapped 18 loci corresponding to previously described genes or
to sequences obtained through homology cloning. The unit maps vary from 774 cM to 1060 cM, with an average value of 14 major
linkage groups. The integrated map is arranged in 17 major linkage groups including 238 loci, plus four small segments with
2–5 marker loci; and covers 1573 cM with an overall average marker interval of 7 cM. Thirty five percent of the markers were
dominant in nature and 30% showed inter-linkage group duplication without any indication of homoeologous linkage groups. Evidence
is provided for the independence of two distinct fertility restoration genes, for the presence of two loosely linked branching
loci, and for marker tightly linked to the Rf1 restoration locus. This map provides an efficient tool in breeding applications such as disease-resistance mapping, QTL analyses
and marker-assisted selection.
Received: 27 August 1998 / Accepted: 28 December 1998 |
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Keywords: | Sunflower Linkage mapping cDNA RFLP Phenotypic traits Composite mapping |
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