Ultrastructural and Cytochemical Identification of Peroxisomes in the Ciliate Ichthyophthirius multifiliis |
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Authors: | ALEXANDRE LOBO-DA-CUNHA CARLOS AZEVEDO |
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Institution: | Department of Cell Biology, Institute of Biomedical Sciences, University of Oporto, 4000 Porto, Portugal |
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Abstract: | ABSTRACT. The peroxisomes of the parasitic ciliate Ichthyophthirius multifiliis were studied, using ultrastructural and cytochemical techniques. In this ciliate most peroxisomes possess a circular or oval section less than 0.6 μm in diameter. However, some dumbbell-shaped and elongated peroxisomes could also be observed. These organelles were frequently associated with the mitochondria and were more abundant in the cell cortex than in the center of the ciliate. Small vesicles and dense nucleoids were usually present in the ultrathin sections of these peroxisomes. Peroxisomal vesicles and tubular structures were selectively impregnated with osmium tetroxide. Catalase was detected by cytochemical techniques in I. multifiliis peroxisomes. |
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Keywords: | Ciliophora enzyme cytochemistry osmium impregnation peroxisomes |
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