Lysed and disrupted Bifidobacterium bifidum BGN4 cells promote anti-inflammatory activities in lipopolysaccharide-stimulated RAW 264.7 cells |
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Authors: | Nayeon Lee Soomin Lee Se-Won Jang Hee Soon Shin Jong-Hyun Park Myeong Soo Park Byung-Hoo Lee |
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Affiliation: | 1. Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of Korea;2. Korea Food Research Institute, Wanju 55365, Republic of Korea;3. Research Center, BIFIDO Co., Ltd., Hongcheon 25117, Republic of Korea |
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Abstract: | Bifidobacterium bifidum BGN4 has been shown to improve the immune system by regulating interleukin (IL)-6 in RAW 264.7 macrophage cells. In this study, the dead cells of B. bifidum BGN4 were produced by enzymatic and physical processing to enhance the inhibition properties of pro-inflammatory cytokines using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Notably, the secretion levels of cytokines such as interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α were decreased by the cell-wall disrupted extracts compared to heat-killed cells. The result suggests that the exposed interior-surface of B. bifidum BGN4 has a potential ability to regulate the immune-responses in the gastrointestinal tract due to major substances in inside-cell wall such as peptidoglycan and teichoic acids. In conclusion, the lysed and disrupted cells from the inside out of B. bifidum BGN4 have anti-inflammatory properties as paraprobiotic agents to control chronic inflammatory related-diseases. |
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Keywords: | Paraprobiotic Anti-inflammation Immunoregulatory |
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