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Designing a highly immunogenic multi epitope based subunit vaccine against Bacillus cereus |
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| Authors: | Muhammad Asif Rasheed Muhammad Awais Adil Aldhahrani Fayez Althobaiti Alaa Alhazmi Sobia Sattar Umara Afzal Hosam Ali Baeshen Hesham Ali El Enshasy Daniel Joe Dailin Ameena A AL-surhanee Faryal Kabir |
| Institution: | 1. Department of Biosciences, COMSATS University Islamabad, Sahiwal Campus, 57000 Sahiwal, Pakistan;2. Department of Biochemistry and Molecular Biology, Faculty of Science, University of Sialkot, Pakistan;3. Clinical Laboratory Sciences Department, Turabah University College, Taif University, Taif 21995, Saudi Arabia;4. Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia;5. Medical Laboratory Technology Department, Jazan University, Jazan, Saudi Arabia;6. SMIRES for Consultation in Specialized Medical Laboratories, Jazan University, Jazan, Saudi Arabia;7. Department of Chemistry, Rawalpindi Women University, Satellite Town Rawalpindi, Pakistan;8. Department of Orthodontics, Faculty of Dentistry, King Abdulaziz University, P. O. Box 80209, Jeddah 21589, Saudi Arabia;9. Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai, Johor, Malaysia;10. School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Skudai, Johor, Malaysia;11. City of Scientific Research and Technology Applications (SRTA), New Burg Al Arab, Alexandria, Egypt;12. Biology Department, College of Science, Jouf University, Sakaka 2014, Kingdom of Saudi Arabia;13. University Institute of Biochemistry and Biotechnology, PMAS-Arid Agriculture University, Rawalpindi, Pakistan |
| Abstract: | ObjectiveSerious non-gastrointestinal-tract infections and food poisoning are caused by Bacillus cereus. Vaccination against B. cereus is very important. The aim of this study was to identify and analyze B and T cell epitopes for chromate transporter protein of the bacteria.MethodsMultiple sequence alignment with the Clustal Omega method was used to identify conserved regions and Geneious Prime was used to produce a consensus sequence. T and B cell epitopes were predicted by various computational tools from the NetCTL and Immune Epitope Database (IEDB), respectively.ResultsAltogether, 6 HTL cells and 11 CTL epitopes were predicted. This vaccine's molecular docking is done with Patch Dock and LigPlot to verify interactions. The immune server (C-IMMSIM) was used to develop In silico immune response in order to assess the multi-epitope vaccine's immunogenic profile.ConclusionWe designed universal vaccine against B. cereus responsible for food poisoning. The disease may be avoided with the aid of the proposed epitope-based vaccine. |
| Keywords: | Epitope-based vaccine Food poisoning Immunity Bioinformatics |
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