Study of optimal conditions for growth and osteogenic differentiation of dental pulp stem cells based on glucose and serum content |
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Authors: | Shilpa Bhandi Ahmed Alkahtani Mohammed Mashyakhy Hosam Ali Baeshen Mohammed Mustafa Hitesh Chohan Nezar Boreak Shankargouda Patil |
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Institution: | 1. Department of Restorative Dental Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia;2. Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia;3. Department of Orthodontics, College of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia;4. Department of Conservative Dental Sciences, College of Dentistry, Prince Sattam Bin Abdulaziz University, Al Kharj, Saudi Arabia;5. Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Saudi Arabia |
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Abstract: | Dental pulp stem cells (DPSCs) have shown promising characteristics in terms of their proliferation and osteogenic differentiation potential, which could be of greater benefit in regenerative dentistry. However, obstacles remain in the in vitro cultivation of DPSCs, which significantly affect their growth and differentiating ability. Therefore in this study, we demonstrated the growth and osteogenic differentiation of DPSCs in the presence of media containing different combinations of serum and glucose to get an optimized combination of both. DPSCs were cultured in media containing combinations of low glucose (LG), low serum (LS), high glucose (HG), and high serum (HS). The proliferation and osteogenic differentiation were assessed in DPSCs cultured with these different combinations of culture conditions. High glucose high serum condition significantly inhibited the proliferation of DPSCs and also affected their clonogenic potential, as evidenced by colony-forming units. Irrespective of the serum content, high glucose in the media also decreased the osteogenic potential of DPSCs confirmed by functional staining, and downregulation of osteogenesis-related genes. High glucose content in the culture media affects the growth and differentiation potential of the DPSCs. Hence, the culture conditions for the DPSCs should be reconsidered to utilize their maximum potential. |
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Keywords: | Dental pulp stem cells Defined culture conditions Differentiation Mesenchymal stem cells DPSCs"} {"#name":"keyword" "$":{"id":"k0030"} "$$":[{"#name":"text" "_":"dental pulp stem cells HGLS"} {"#name":"keyword" "$":{"id":"k0040"} "$$":[{"#name":"text" "_":"high glucose low serum HGHS"} {"#name":"keyword" "$":{"id":"k0050"} "$$":[{"#name":"text" "_":"high glucose high serum LGLS"} {"#name":"keyword" "$":{"id":"k0060"} "$$":[{"#name":"text" "_":"low glucose low serum LGHS"} {"#name":"keyword" "$":{"id":"k0070"} "$$":[{"#name":"text" "_":"low glucose high serum |
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