Partially purified actinomycetes compounds enhance the intracellular damages in multi-drug resistant P. aeruginosa and K. pneumoniae |
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Institution: | 1. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;2. Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India;3. Department of Nanotechnology, Noorul Islam University, Kumaracoil 629180, Tamil Nadu, India |
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Abstract: | Based on the excellent nutrient level, the current study was focused on isolation and anti-bacterial activity of the actinomycetes from marine mangrove soil samples. As result, 10 different strains of actinomycetes strains were identified on actinomycetes isolation agar plates. The identified strains were shown with white, clear, uncontaminated well matured spore producing ability. Based on the initial observation, the isolated colonies were actinomycetes. The partially extracted crude compound shown excellent anti-bacterial activity against P. aeruginosa and K. pneumoniae with 15 mm and 13 mm zone of inhibitions were observed at 500 μL concentrations. The minimum inhibition concentration result was also confirmed the 500 μL concentration against both the tested concentration with high inhibition rate. Then, the intracellular damages, decreased cell growth of the crude actinomycetes extract treated bacterial strains were clearly observed by confocal laser scanning electron microscope. The extracellular damages of bacterial cell wall and shape of the both the pathogens were clearly shown by scanning electron microscope. Therefore, all the results were clearly supported to the partially extracted crude compound and it has excellent anti-bacterial activity against tested multi drug resistant bacteria. |
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Keywords: | Marine mangrove soil Actinomycetes Biomedical application Intracellular damage Scanning electron microscope |
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