Age-related changes associated with the induction of o-pyrocatechuic acid carboxylyase in Aspergillus ornatus

Mycelial mats of Aspergillus ornatus grown on cellulose xanthate membranes placed on a defined agar medium showed o-pyrocatechuic acid carboxyalse activity which could be induced to over six times its basal level by the addition of 0.1% L-tryptophan to the medium. Exposure of mature mycelial mats to cyclohexamide prior to induction indicated that de novo protein synthesis was required for this induction to occur. The effect of age of this enzyme adaptation process was investigated by transferring membranes and mats of variuos ages from defined medium to fresh defined medium plus 0.1% L-tryptophan With increasing mycelial age, the basal enzyme activity level remained relatively unchanged while the level to which the enzyme could be induced decreased significantly. This decrease was linear when plotted against the mean cell age of mycelium, conforming to a general model of age-dependent modifications of the enzyme adaptation process proposed by Adelman. Several possible explanations of this phenomenon are discussed.