Azo dye decolorization with a mutant Escherichia coli strain |
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Authors: | Jo-Shu Chang Tai-Shin Kuo Yun-Peng Chao Jin-Yen Ho Ping-Jei Lin |
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Affiliation: | (1) Department of Chemical Engineering, Feng Chia University, Taichung, Taiwan, R.O.C. |
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Abstract: | A recombinant Escherichia coli strain (E. coli NO3) containing genomic DNA fragments from azo-reducing wild-type Pseudomonas luteola strain decolorized a reactive azo dye (C.I. Reactive Red 22) at approx. 17 mg dye h–1 g cell. The ability to decolorize the azo dye probably did not originate from the plasmid DNA. Acclimation in azo-dye-containing media gave a nearly 10% increase in the decolorization rate of E. coli NO3. Growth with 1.25 g glucose l–1 completely stopped the decolorization activity. When the decolorization metabolites from E. coli NO3 were analyzed by HPLC and MS, the results suggested that decolorization of the azo dye may be due to cleavage of the azo bond. |
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Keywords: | azo dye decolorization Escherichia coli |
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