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Hypoxia affects in vitro proliferation and differentiation of mouse corneal epithelial progenitor cell |
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| Authors: | Nuo Dong Wenjuan Qin Yuhua Xue Cheng Li Zuguo Liu |
| Institution: | 1. Eye Institute and Affiliated Xiamen Eye Center of Xiamen University, Xiamen, Fujian, 361005, China 2. Affiliated Zhongshan Hospital of Xiamen University, Xiamen, Fujian, 361001, China 3. School of Pharmaceutical Sciences Xiamen University, Xiamen, Fujian, 361005, China 4. Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Eye Institute of Xiamen University, Medical College of Xiamen University, Xiamen University Affiliated Xiamen Eye Center, 168 Daxue Road, Xiamen, Fujian, 361005, China |
| Abstract: | This study was to investigate the proliferation and differentiation of mouse corneal epithelial progenitor cell in hypoxic airlift culture. Mouse corneal epithelial progenitor cell line progenitor cells were cultured under airlift with normoxic and hypoxic conditions for various durations up to 2 wk. Under normoxic conditions when exposed to air, the hyperproliferation and abnormal epidermal-like differentiation of mouse corneal epithelium was induced, whereas when exposed to air under hypoxic conditions, although we observed augmented proliferation, the abnormal differentiation was inhibited. The mechanism by which hypoxia prevents abnormal differentiation may involve downregulation of Wnt signaling pathways, which were inhibited in cells cultured with hypoxic airlift technique. In conclusion, hypoxia can prevent abnormal differentiation while enhancing the proliferation of corneal epithelial cells by blocking Wnt/β-catenin signaling pathway. |
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