Differential sensitivity of human normal and malignant cells to 8-azahypoxanthine in vitro |
| |
| Authors: | M H Vaughan M W Steele |
| |
| Affiliation: | 1. Department of Biochemistry, Faculty of Arts and Sciences, University of Pittsburgh, Pittsburgh, Pa 15213, USA;2. Department of Pediatrics, Childrens Hospital of Pittsburgh, and University of Pittsburgh School of Medicine, Pittsburgh, Pa 15213, USA;1. School of Medicinal and Chemical Engineering, Yangling Vocational & Technical College, Yangling 712100, PR China;2. Shaanxi Key Labotory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, PR China;1. Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-sen University, 510006 Guangzhou, Guangdong, PR China;2. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, 510060 Guangzhou, Guangdong, China;1. Department of Clinical Laboratory Medicine, The Cancer Hospital of Shantou University Medical College, Shantou 515041, PR China;2. Department of Biochemistry and Molecular Biology, Shantou University Medical College, No. 22, Xinling road, Shantou 515041, PR China;3. Cancer Research Lab, The Cancer Hospital of Shantou University Medical College, Shantou 515041, PR China;4. Department of Surgical Oncology, Shantou Central Hospital, Shantou 515041, PR China;5. Department of Dermatology and Venereology, Shantou Central Hospital, Shantou 515041, PR China;6. Institute of Oncologic Pathology, Shantou University Medical College, Shantou 515041, PR China;1. Section of Endocrinology, Nutrition, and Diabetes, Department of Medicine, Boston University Medical Center, Boston, MA |
| |
| Abstract: | Human fibroblast strains in vitro are markedly resistant to medium containing levels of 8-azahypoxanthine (AH) which are lethal to malignant cells. This difference depends on the presence of hypoxanthine in the fetal calf serum used to make standard tissue culture medium. Although malignant cells in culture possess approximately three times more effective hypoxanthine-guanine phosphoribosyl transferase (HGPRT) activity than normal cells, this does not account for the protective action of hypoxanthine in the latter. Rather, our data, together with that of others, suggest that hypoxanthine protects normal cells from AH by preventing its reaction with phosphoribosyl pyrophosphate via HGPRT. Whether this is the consequence of a qualitative difference between the HGPRT's of malignant and normal cells or a reflection of differential permeability to hypoxanthine requires further experimentation. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
