Reference material for the evaluation of a standard method for the detection of salmonellas in foods and feeding stuffs |
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| Authors: | H J Beckers FM van Leusden M J M Meijssen † EH Kampelmacher |
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| Institution: | *Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Hygiene, P.O. Box 1, 3720 BA Bilthoven;The Netherlands and Laboratory for Food Microbiology and Hygiene, Agricultural University Biotechnion, De Dreyen 12, 6703 BC Wageningen, The Netherlands;?University of Leiden, Department of Infectious Diseases, Rijnsburgerweg 10, 2333 AA Leiden, The Netherlands |
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| Abstract: | To evaluate a standard salmonella isolation method a reference material consisting of 0.2 g spray-dried milk inoculated with Salmonella typhimurium and contained in gelatin capsules was prepared. The organisms were distributed homogeneously between capsules, and their numbers were stable for 120 d when the capsules were stored in dry conditions at 4 C. Addition of these capsules with or without food samples to pre-enrichment broth gave low and reproducible levels of Salm. typhimurium contamination without altering the pre-enrichment and without influencing the other bacterial flora present. As a result of an interlaboratory trial, the reference material indicated that the food and/or its competitive flora may have a negative influence on the detection of salmonellas. |
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