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| 苜蓿中华根瘤菌matB基因的克隆及其功能的研究 | |
| 引用本文： | 王永宝,孙杰,刘君,陈爱民,王彦章.苜蓿中华根瘤菌matB基因的克隆及其功能的研究[J].微生物学杂志,2009,29(4):1-5. |
| 作者姓名： | 王永宝 孙杰 刘君 陈爱民 王彦章 |
| 作者单位： | 1. 石河子大学,生命科学学院,新疆,石河子,832000;石河子大学,新疆兵团绿洲生态农业重点实验室,新疆,石河子,832003 2. 石河子大学,新疆兵团绿洲生态农业重点实验室,新疆,石河子,832003 3. 新疆农业大学,林学院,新疆,乌鲁术齐,830052 4. 中国科学院,上海生命科学研究院植物生理生态研究所,上海,200032 |
| 基金项目： | 国家自然科学基金项目，上海市科委国内合作项目 |
| 摘 要： | 通过同源性分析，发现苜蓿中华根瘤茵（Sinorhizobium meliloti）菌株Rm1021中matB基因与三叶草生物型豌豆根瘤茵（Rhizobium leguminosarum by．trifolii）和慢生型大豆根瘤茵（Bradyrhizobium japonicum USDA110）中编码丙二酸单酰辅酶A合成酶（malonyl-CoA synthetase）基因在氨基酸水平上分别达到了75％和67％一致性，具有高度同源性。因此，从Rml021中克隆出matB基因，并在大肠埃希菌（Escherichiacoli）中进行体外诱导表达和纯化。纯化的MatB蛋白具有丙二酸单酰辅酶A合成酶的活性，测定的Km值是710μmol，Vmax是0．209μmol／min／mg。 |
| 关 键 词： | 苜蓿中华根瘤菌 matB 丙二酸单酰辅酶A合成酶 酶活力 |
Cloning and Functional Analysis of matB Gene in Sinorhizobium meliloti |
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| WANG Yong-bao,SUN Jie,LIU Jun,CHEN Ai-min,WANG Yan-zhang.Cloning and Functional Analysis of matB Gene in Sinorhizobium meliloti[J].Journal of Microbiology,2009,29(4):1-5. | |
| Authors: | WANG Yong-bao SUN Jie LIU Jun CHEN Ai-min WANG Yan-zhang |
| Institution: | WANG Yong-bao, SUN Jie, LIU Jun, CHEN Ai-min, WANG Yan-zhang(1. Department of llfe science, Shihe：i University, Shihezi, Xinfiang 832000, China; 2. The key Oasis Eco-agricultztre Laboratory of Xinjiang Production and Construction Group. Shihezi University. Shihezi. Xinjiang 832003, Chino 3. College of Forestry and Horticulture. Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China 4. National Laboratory of Plant Molecular Genetics, htstitate of Plant Physiology ＆ Ecology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. |
| Abstract: | Based on the analysis of amino acids homology, we found that matB gene in Sinorhizobium meliloti Rml021 show highly homology with the malonyl-CoA synthetase of the Rhizobium leguminosarum by. trifolii and the Bradyrhizobium japonicttm USDA 110. The MatB protein shares 75% and 67% indentity with malonyl-CoA synthetase of the R. leguminosarum by. trifolii and the B. japonicurn USDA I I0, respectively. So we cloned matB gene, constructed the matB recombinant plasmid and purified it from E. coll. We found that the purified MatB have the activity of malonyl-CoA synthetase, showing that its Kin value is 710 μmol and Vmax is 0. 209 μxmol/min/mg. |
| Keywords: | matB |
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