| 香菇栽培种线粒体DNA和核糖体DNA多态性研究初探 |
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| 引用本文： | 陈春涛,张引芳.香菇栽培种线粒体DNA和核糖体DNA多态性研究初探[J].微生物学杂志,1997,17(2):23-29. |
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| 作者姓名： | 陈春涛 张引芳 |
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| 作者单位： | 郑州轻工学院食工系!450002(陈春涛)，上海农科院食用菌所!201106(张引芳)，河南农大生物工程学院!450002(姚占芳)，济南军区血研所!463000(周文丽) |
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| 摘 要： | 本文应用RFLP技术研究了10个香菇主栽品种的线粒体DNA（mtDNA）和核糖体DNA（rDNA）的部分小区段，利用PCR技术扩增了rDNA5．8＋ITS区段及mtDNA的小区段，分析这些片段的限制性酸切图谱，并进行菌株间的遗传相似系数的估算。结果显示：菌株间的rDNA在5．8＋ITS区段差异很小，表明同一种内菌株间的rDNA具有相对的遗传稳定性；不同菌株间未检出mtDNA的差异，表明菌株间在所研究的区段具有很高的遗传相似性。
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| 关 键 词： | 香菇 核糖体DNA 线粒体DNA RFLP |
INITIAL STUDY ON RIBOSOMAL DNA AND MITOCHONDRIAL DNA POLYMORPHISMS OF CULTIVATE OF LENTINUS EDODES |
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| Chen Chuntao,et al..INITIAL STUDY ON RIBOSOMAL DNA AND MITOCHONDRIAL DNA POLYMORPHISMS OF CULTIVATE OF LENTINUS EDODES[J].Journal of Microbiology,1997,17(2):23-29. |
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| Authors: | Chen Chuntao |
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| Institution: | Chen Chuntao, et al. |
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| Abstract: | Restriction polymorphisms in parts region of ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) were examined in 10 culti-vated strains of L.edodes. The PCR was used to seperately amplify the 5.8s rDNA and the intemal transcribed spacers (ITS), and a region of mtDNA. Amplified products were digested With special restriction endonucleases and the data were analysed with NTSYS program. The results show that: There is very low degree of variation in rDNA. It suggests that rDNA is relatively stable within strains in the same spe-cies. There is no variation in mtDNA within these strains. It indicates that there is a very high degree of genetic similarity within strains in these mtDNA regions. |
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| Keywords: | Xianggu mushroom (L edodes) ribosomal DNA mitochondrial DNA RFLP |
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