Binding and photocleavage of cationic porphyrin-phenylpiperazine hybrids to DNA |
| |
Authors: | Jia Tao Jiang Zhong-Xing Wang Kai Li Zao-Ying |
| |
Institution: | College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, Hubei, PR China. |
| |
Abstract: | The binding properties of cationic porphyrin-phenylpiperazine hybrids to calf thymus (CT) DNA were investigated by using absorption, fluorescence and circular dichroism (CD) spectra, and the apparent affinity binding constants (K(app)) of the porphyrins for CT DNA were determined by using a competition method with ethidium bromide (EB). Intercalation of porphyrin into CT DNA occurred when two phenylpiperazines were introduced at cis position onto the periphery of cationic porphyrin. The photocleavages of pBR322 plasmid DNA by the porphyrins were consistent with the values of K(app). With porphyrin]/DNA base pairs] ratio increased, the binding mode tended to be outside binding, and the cleavage abilities of the porphyrins varied. In the presence of sodium azide, a quencher of 1O2, the cleavage of DNA by the porphyrin of intercalation was less inhibited. |
| |
Keywords: | Cationic porphyrin Phenylpiperazine Intercalation Apparent affinity binding constant Photocleavage |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|