Triacylglycerol-rich lipoproteins trigger the phosphorylation of extracellular-signal regulated kinases in vascular cells |
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Authors: | Pacheco Yolanda M Abia Rocío Perona Javier S Meier Kathryn E Montero Emilio Ruiz-Gutiérrez Valentina Muriana Francisco J G |
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Institution: | Instituto de la Grasa, CSIC, Avda. Padre García Tejero 4, 41012 Seville, Spain. |
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Abstract: | Postprandial triacylglycerol-rich lipoproteins (TRL) have been implicated in the pathophysiology of atherosclerosis, but the intracellular processes by which TRL could affect vascular function are still unknown. Incubation of TRL obtained at 2 h postprandial period with vascular smooth muscle cells (VSMC) produced a tyrosine phosphorylation of the extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2) that belong to the mitogen-activated protein kinase (MAPK) family. The activation of ERK1 and ERK2 had a maximum at 15 min, returned to baseline by 60 min, and was partially depleted after incubation of cells with a MAPKK inhibitor (PD 098059). In addition, postprandial TRL did competent VSMC for DNA replication through a MAPK pathway. These effects were dependent of the lipid composition of TRL. Our observations suggest that postprandial TRL can trigger activation of the MAPK pathway and induce a mitogenic response in VSMC in a lipid-dependent fashion. |
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Keywords: | Triacylglycerol-rich lipoproteins Vascular cells Mitogen-activated protein kinase Proliferation Atherosclerosis |
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