| 土壤中棉花黄萎病菌SYBR Green Ⅰ荧光RT-PCR定量检测技术研究 |
| |
| 引用本文： | 肖蕊,余真真,Elsharawy AA,魏锋,杨家荣.土壤中棉花黄萎病菌SYBR Green Ⅰ荧光RT-PCR定量检测技术研究[J].菌物学报,2011,30(4). |
| |
| 作者姓名： | 肖蕊 余真真 Elsharawy AA 魏锋 杨家荣 |
| |
| 作者单位： | 西北农林科技大学植物保护学院,陕西省农业分子生物学重点实验室,杨凌,712100 |
| |
| 基金项目： | 国家公益性行业(农业)科研专项(3-19); 高等学校学科创新引智计划项目(No.B07049) |
| |
| 摘 要： | 为实现田间土壤棉花黄萎病菌的早期检测,建立了土壤中棉花黄萎病菌的SYBR Green I荧光定量PCR检测方法。以含342bp PCR扩增产物的阳性质粒为参考,构建了标准曲线,并对该曲线的特异性、敏感性、可重复性进行了评价。结果表明,该方法具有快速、特异性强、敏感度高等特点。检测范围在3.8×103-3.8×108copies/μL之间有良好的线性关系,相关系数R2为0.996,扩增效率为101.5%,灵敏度比常规PCR方法高102倍。
|
| 关 键 词： | 大丽轮枝菌 SYBR Green I 荧光实时定量 |
SYBR Green Ⅰ real time RT-PCR assay for quantitatively detecting the occurrence of Verticillium dahliae of cotton in naturally infested soil |
| |
| Authors: | XIAO Rui YU Zhen-Zhen Elsharawy AA WEI Feng YANG Jia-Rong |
| |
| Institution: | XIAO Rui YU Zhen-Zhen Elsharawy AA WEI Feng YANG Jia-Rong College of Plant Protection of Northwest Agriculture & Forestry University and Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling 712100,China |
| |
| Abstract: | In order to explore early detection the occurrence of Verticillium dahliae of cotton in naturally infested soil,a SYBR Green I based Real time RT-PCR assay was developed.The positive plasmid included a 342bp PCR product was used as the reference and the specification curve was created on that basis.The specificity,sensitivity and reproducibility of the curve were evaluated and compared with conventional PCR techniques.The result showed that the Real time RT-PCR assay was rapid,highly sensitive and specific.... |
| |
| Keywords: | Verticillium dahliae SYBR Green I Real time RT-PCR assay |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《菌物学报》浏览原始摘要信息 |
| 点击此处可从《菌物学报》下载免费的PDF全文 |
