| 骨髓瘤细胞中阿霉素诱导的ATP 变化与自噬的关系 |
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| 引用本文： | 余芳陈协群 陈娟娟黄晓梅. 骨髓瘤细胞中阿霉素诱导的ATP 变化与自噬的关系[J]. 现代生物医学进展, 2011, 11(6): 1030-1032 |
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| 作者姓名： | 余芳陈协群 陈娟娟黄晓梅 |
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| 作者单位： | 第四军医大学西京医院血液内科,陕西,西安,710032 |
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| 摘 要： | 目的:检测用阿霉素(doxorubicin DOXO)处理的骨髓瘤细胞株NCI-H929中ATP与自噬表达水平的变化,探讨两者之间的关联。方法:分别以DOXO 2umol/l 24h、DOXO 2umol/l联用自噬抑制剂3MA 10mmol/l 24h处理H-929细胞后,采用MTT法检测细胞存活率;ATP生物发光法检测ATP表达量;Western Blot检测靶细胞自噬标志分子LC3蛋白的表达。结果:各组相对未处理组存活率分别为54%、35%;相对未处理组%ATP分别为400%、150%;DOXO 24h LC3表达显著上调。结论:经DOXO处理H-929细胞系自噬形成,进而ATP上升以保护细胞。
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| 关 键 词： | 阿霉素 骨髓瘤 三磷酸腺苷 自噬 NCI-H929细胞 LC3 |
Relationship between ATP and Autophagy in Myeloma Cell LinesNCI-H929 Induced by Doxorubicin |
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| YU Fang,CHEN Xie-qun,CHEN Juan-juan,HUANG Xiao-mei. Relationship between ATP and Autophagy in Myeloma Cell LinesNCI-H929 Induced by Doxorubicin[J]. Progress in Modern Biomedicine, 2011, 11(6): 1030-1032 |
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| Authors: | YU Fang CHEN Xie-qun CHEN Juan-juan HUANG Xiao-mei |
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| Affiliation: | YU Fang,CHEN Xie-qun,CHEN Juan-juan,HUANG Xiao-mei(Department of Hematology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China) |
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| Abstract: | Objective:As a major intracellular degradation system that is found ubiquitously in eukaryotes,autophagy plays a key role in maintaining protein metabolic equilibrium,cell homeostasis,regulating cellular constituent recycling,cell development and differ-entiation.In different cell types or different stress modes,the role or function of autophagy changes.What's the function of autophagy in myeloma cells? We don't have experience at present.Based on the background above,in this study,by means of the model of ... |
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| Keywords: | LC3 |
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