Individual human tumors in short-term micro-organ cultures: Chemosensitivity testing by fluorescent cytoprinting |
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Authors: | Boris Rotman Carl Teplitz Katherine Dcckinson Joseph P Cozzolino |
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Institution: | (1) Division of Biology and Medicine, Brown University, 02912 Providence, Rhode Island |
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Abstract: | Summary Using innovative approaches, we addressed several problems often associated within vitro chemosensitivity testing of individual human tumors: 1) obtaining a high rate of evaluability; 2) excluding participation
of nonmalignant stromal and vascular components usually present in tumor specimens; 3) preserving cell-to-cell interactions
present in the original tumor; 4) assessing drug-induced cytotoxicity without sacrificing the tumor culture. To circumvent
these problems, tumor specimens were processed as follows: i) tissue (fresh or cryopreserved) was mechanically or enzymatically
dissociated under mild conditions into cellular clusters (termed micro-organs); ii) large micro-organs were separated by a
brief decantation, resuspended, and then exposed to fluorescein acetate to visualize (under naked eye) viable micro-organs;
iii) fluorescent (i.e., viable) micro-organs were collected using a Pasteur pipette, and then planted on a solid support made
of cellulose fibers impregnated with collagen. Since tumor micro-organs have been previously shown to consist solely of malignant
cells, the procedure described here not only preserves a critical portion of the tumor architecture but eliminates at the
onset necrotic tissue and nonmalignant cellular components that could interfere with the chemosensitivity testing. Drug-induced
cytotoxicity was measured by “fluorescent cytoprinting”, a novel, nondestructive procedure for assessing micro-organ viabilityin situ. The key feature of fluorescent cytoprinting is that cytotoxic effects arenot measured against control cultures but against a baseline provided by a cytoprint of the same culture before drug addition.
Using three experimental designs, we tested the potential of the method for clinical applications. The results using 469 human
malignant tumors showed that the micro-organ culture assay can distinguish individual tumor chemosensitivity profiles with
an overall success rate of 96%. For three commonly used chemotherapeutic drugs, the observed frequency of responding tumors
was found to be comparable to previously reported clinical results using single agents.
This work was partially supported by Brown University Research Foundation. |
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Keywords: | human tumor in vitro chemotherapy organ culture cytotoxicity |
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