Expression of the threonine operon fromEscherichia coli inBrevibacterium flavum andCorynebacterium glutamicum期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Expression of the threonine operon fromEscherichia coli inBrevibacterium flavum andCorynebacterium glutamicum

Authors:	M. Pátek O. Navrátil J. Hochmannová J. Nešvera J. Hubáček

Affiliation:	(1) Institute of Microbiology, Czechoslovak Academy of Sciences, Vídeská 1083, CS-142 20 Praha 4, Czechoslovakia

Abstract:	Summary The threonine operon fromEscherichia coli was cloned in plasmid pBR322, subcloned into the shuttle vector pCEM300 and the resulting recombinant plasmid was transferred intoBrevibacterium flavum andCorynebacterium glutamicum. The expression ofE. coli threonine genes in these coryneform bacteria was demonstrated by complementing thethrA andthrB mutations and by assaying homoserine dehydrogenase activity.

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