Isolation and characterization of Kit-independent melanocyte precursors induced in the skin of Steel factor transgenic mice |
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Authors: | Kawaguchi Atsushi Chiba Kairi Tanimura Yu Motohashi Tsutomu Aoki Hitomi Takeda Tomoko Hayashi Shin-Ichi Shimizu Katsuji Kunisada Takahiro |
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Affiliation: | Department of Tissue and Organ Development, Regeneration, and Advanced Medical Science,;Department of Orthopaedic Surgery, Gifu University Graduate School of Medicine, Gifu 501-1194,;Division of Immunology, Department of Molecular and Cellular Biology, School of Life Science, Faculty of Medicine, Tottori University, Yonago 683-8503, Japan |
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Abstract: | Steel factor (SLF, also called KIT-ligand, mast cell growth factor, or stem cell factor) acting through the tyrosine kinase receptor KIT is thought to be indispensable for the early phase of melanocyte development both in vivo and in vitro . In the present study, Kit-independent precursor cells were generated in mice expressing exogenous SLF in their skin keratinocytes and were detected as pigmented spots after administration of Kit function-blocking antibody. We successfully purified these precursor or stem cells as Kit+CD45− cells by flow cytometry. The purified cells showed normal but delayed differentiation into mature melanocytes, indicating the immature nature of Kit-independent precursors. The Kit-independent interfollicular population generated in SLF transgenic mice was suggested to be the counterpart of the follicular melanocyte stem cell based on the Kit-independent nature for their survival. |
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Keywords: | Kit melanocyte precursors melanocyte stem cells melanocytes stem cell factor stem cell factor transgenic mouse |
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