Characterization of a Vibrio cholerae phage isolated from the coastal water of Peru |
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| Authors: | Talledo Miguel Rivera Irma N G Lipp Erin K Neale Angela Karaolis David Huq Anwar Colwell Rita R |
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| Affiliation: | Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 701 E. Pratt St., Baltimore, Maryland 21202, USA.;Microbiology and Microbial Biotechnology Laboratory, Faculty of Biological Sciences, Universidad Nacional Mayor de San Marcos, Lima, Perú.;Department of Microbiology, Biomedical Science Institute, University of São Paulo, CEP 05508–900, S. Paulo, Brazil.;Department of Environmental Health Science, University of Georgia, Athens, Georgia 30602, USA.;University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.;Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742, USA. |
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| Abstract: | A Vibrio cholerae bacteriophage, family Myoviridae, was isolated from seawater collected from the coastal water of Lima, Peru. Genome size was estimated to be 29 kbp. The temperate phage was specific to V. cholerae and infected 12/13 V. cholerae O1 strains and half of the four non-O1/non-O139 strains tested in this study. Vibrio cholerae O139 strains were resistant to infection and highest infection rates were obtained in low nutrient media amended with NaCl or prepared using seawater as diluent. |
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