| 改造中国仓鼠卵巢细胞 |
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| 引用本文： | 来大志 齐连权 于长明 王海涛,陈薇.改造中国仓鼠卵巢细胞[J].生物工程学报,2002,18(4):415-419. |
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| 作者姓名： | 来大志 齐连权 于长明 王海涛 陈薇 |
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| 作者单位： | 北京微生物流行病研究所,北京,100071 |
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| 摘 要： | 原核细胞、酵母细胞以及昆虫细胞相比，中国仓鼠卵巢细胞（CHO）作为宿主细胞表达的外源蛋白最接近其天然构象，因而CHO细胞表达系统是生物工程制药最为理想的表达系统。但这种系统也存在诸多缺点。如在大规模培养中CHO细胞会面临着对无血清培养基的适应性差、细胞无限度增殖以及细胞凋亡等很多难题。所以除了在培养基、培养条件和表达载体方面下功夫优化该系统外，对CHO细胞本身进行改造已成为优化CHO表达系统的另一热点。
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| 关 键 词： | CHO细胞 改造， 大规模培养， 细胞凋亡， 细胞黏附， 细胞周期 |
| 文章编号： | 1000-3061(2002)04-0415-05 |
| 修稿时间： | 2002年1月10日 |
Modification of Chinese Hamster Ovary Cells |
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| LAI Da-Zhi,QI Lian-Quan,YU Chang-Ming,WANG Hai-Tao,CHEN Wei.Modification of Chinese Hamster Ovary Cells[J].Chinese Journal of Biotechnology,2002,18(4):415-419. |
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| Authors: | LAI Da-Zhi QI Lian-Quan YU Chang-Ming WANG Hai-Tao CHEN Wei |
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| Institution: | Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. dzlai@yahoo.com |
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| Abstract: | Chinese hamster ovary cells (CHO) are preferable to prokaryotic, yeast or insect cells as hosts for biopharmaceutical production due to the products are more similar to their natural conformation. However, CHO cells confront tremendous difficulties when cultured in large scale such as mal-adaptation to serum-free medium, apoptosis and over-growth without limitation. So in addition to optimizing CHO system in respect of medium, environment and expression vector, modification of CHO cells themselves has drawn more and more attention. Here the main progress in CHO-modification is reviewed. |
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| Keywords: | CHO cells large-scale culture apoptosis cell-adhesion cell cycle |
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