| 转金属硫蛋白αα突变体基因的矮牵牛对铅的抗性及积累的研究 |
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| 引用本文： | 李伟,张竞,张晓钰,单龙,茹炳根. 转金属硫蛋白αα突变体基因的矮牵牛对铅的抗性及积累的研究[J]. 生物化学与生物物理进展, 2001, 28(3): 405-409 |
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| 作者姓名： | 李伟 张竞 张晓钰 单龙 茹炳根 |
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| 作者单位： | 北京大学生命科学学院, |
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| 基金项目： | “863”计划资助项目(863-103-21-09). |
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| 摘 要： | 利用农杆菌介导的方法将MT及αα基因导入矮牵牛中.转基因植株对铅(Pb)的抗性及吸收能力明显高于对照.转MT及αα基因的植株分别能在150及200 μmol/L Pb中正常生长，而对照只能在50 μmol/L Pb中正常生长.转MT及αα基因植株后代种子萌发与对照相比表现出明显的抗Pb优势.Pb的吸收实验表明转MT、αα基因的植株对Pb的吸收分别比对照提高了28%和35%.
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| 关 键 词： | 金属硫蛋白，重组体蛋白αα，转基因矮牵牛，铅，抗性，吸收 |
| 收稿时间： | 2000-07-13 |
| 修稿时间： | 2000-09-28 |
Pb Tolerance and Accunulation of Petunia Transformed by Metallothionein Recombinant αα Gene |
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| LI Wei,ZHANG Jing,ZHANG Xiao-Yu,SHAN Long and RU Bing-Gen. Pb Tolerance and Accunulation of Petunia Transformed by Metallothionein Recombinant αα Gene[J]. Progress In Biochemistry and Biophysics, 2001, 28(3): 405-409 |
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| Authors: | LI Wei ZHANG Jing ZHANG Xiao-Yu SHAN Long RU Bing-Gen |
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| Affiliation: | The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China;The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China;The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China;The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China;The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China |
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