Selective labeling of pulmonary surfactant protein SP-C in organic solution |
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Authors: | Plasencia I Cruz A López-Lacomba J L Casals C Pérez-Gil J |
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Institution: | Departamento de Bioquímica, Facultad de Biología, Madrid, 28040, Spain. |
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Abstract: | Pulmonary surfactant protein SP-C has been isolated from porcine lungs and treated with dansyl isothiocyanate in chloroform:methanol 2:1 (v/v) solutions,under conditions optimized to introduce a single dansyl group covalently attached to the N-terminalamine group of the protein without loss of its native thioesther-linked palmitic chains. The resulting derivative Dans-SP-C conserves the secondary structure of native SP-C as well as the ability to promote interfacial adsorption of DPPC suspensions and to affect the thermotropic behavior of DPPC bilayers. This derivative can be used to characterize lipid-protein and protein-protein interactions of a native-like SP-C in lipid/protein complexes. |
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