Selective enrichment of Ser-/Thr-phosphorylated peptides in the presence of Ser-/Thr-glycosylated peptides |
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| Authors: | Poot Alex J Ruijter Eelco Nuijens Timo Dirksen Eef H C Heck Albert J R Slijper Monique Rijkers Dirk T S Liskamp Rob M J |
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| Affiliation: | Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands. |
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| Abstract: | Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these conditions to achieve a beta-elimination that is highly selective for phosphorylated peptides. This is the first report of selective beta-elimination and enrichment of phosphorylated peptides in the presence of glycosylated peptides. |
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| Keywords: | Chemoselectivity β‐Elimination Glycoproteomics MALDI‐TOF MS Phosphoproteomics |
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