UHRF1 regulates CDH1 via promoter associated non-coding RNAs in prostate cancer cells |
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Authors: | Elena Magnani Filippo Macchi Monica Mancini Vanessa Lomazzi Sara Cogliati Christian Pistore Martina Mandruzzato Anne-Catherine Dock-Bregeon Ian Marc Bonapace |
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Institution: | 1. Department of Biotechnology and Life Sciences, University of Insubria, 21052 Busto Arsizio, VA, Italy;2. IGBMC, BP10142, 1 rue Laurent Fries, 67404 Illkirch Cedex, France |
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Abstract: | Non-coding RNAs (ncRNAs) transcribed from the promoter and the downstream region can affect the expression of the corresponding coding genes. It has been shown that sense-directed ncRNAs arising from the promoter region of the E-cadherin gene (CDH1) mediate its repression. Here, we show that an antisense-directed ncRNA (paRCDH1-AS) transcribed from the CDH1 promoter is necessary for its expression. paRCDH1-AS acts as a hooking scaffold by recruiting the epigenetic regulators, UHRF1, DNMT3A, SUV39H1 and SUZ12, involved in CDH1 repression. The binding of epigenetic regulators to paCRDH1-AS, indeed, prevents their localization to the chromatin on CDH1 promoter. Moreover, paRCDH1-AS silencing induces CDH1 repression and a switch of the epigenetic profile on the promoter towards a more closed chromatin. Using bioinformatic and experimental approaches we defined that the promoter of the paRCDH1-AS is shared with the E-cadherin gene, showing a bidirectional promoter activity. We found that UHRF1 controls both CDH1 and paRCDH1-AS by directly binding this bidirectional promoter region. Our study provides evidences, for the first time, that UHRF1 recruitment can be affected by promoter-associated non-coding RNAs, opening new perspective regarding the role of UHRF1 in these complex regulatory networks. |
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Keywords: | Promoter-associated non-coding RNAs UHRF1 CDH1 Bidirectional promoters |
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