The role of blood clotting factor V in the conversion of prothrombin and a decarboxy prothrombin into thrombin |
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Authors: | C Vermeer J W Govers-Riemslag B A Soute M J Lindhout J Kop H C Hemker |
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Abstract: | Purified PIVKA-II exhibits some factor II (prothrombin) activity in the one-stage coagulation assay and this factor II activity does not come from residual amounts of factor II but originates from PIVKA-II itself. It is shown that PIVKA-II is converted by a normal prothrombinase complex (factor Va and factor Xa adsorbed onto a phospholipid interface) more readily than by phospholipids and factor Xa alone. This suggests that binding between PIVKA-II and factor Va is an essential feature in the formation of the enzyme . substrate complex and from this we infer that a direct interaction between factor Va and prothrombin plays a r?le in the prothrombinase . prothrombin complex. |
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