Transglucosylation of tertiary alcohols using cassava beta-glucosidase |
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Authors: | Svasti Jisnuson Phongsak Thanawat Sarnthima Rakrudee |
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Institution: | Center for Excellence in Protein Structure and Function, Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand. scjsv@mahidol.ac.th |
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Abstract: | We have compared the ability of beta-glucosidases from cassava, Thai rosewood, and almond to synthesize alkyl glucosides by transglucosylating alkyl alcohols of chain length C(1)-C(8). Cassava linamarase shows greater ability to transfer glucose from p-nitrophenyl-beta-glucoside to secondary alcohol acceptors than other beta-glucosidases, and is unique in being able to synthesize C(4), C(5), and C(6) tertiary alkyl beta-glucosides with high yields of 94%, 82%, and 56%, respectively. Yields of alkyl glucosides could be optimized by selecting appropriate enzyme concentrations and incubation times. Cassava linamarase required pNP-glycosides as donors and could not use mono- or di-saccharides as sugar donors in alkyl glucoside synthesis. |
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Keywords: | β-Glucosidase Alkyl glucoside Tertiary alcohol Transglucosylation Linamarase Cassava Manihot esculenta pNP-glucopyranoside |
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