| Abstract: | Two of five opossums examined from the Kansas City area were found to be infected with Besnoitia darlingi. Inoculation of cysts resulted in acute lethal infections in mice and hamsters. Chronic infections with development of tissue cysts were obtained in mice by prophylaxis with sulfadiazine. Cysts were fed to cats and a dog which resulted in the shedding of isosporoid oocysts by cats. The prepatent period was 11 to 14 days. Unsporulated oocysts averaged 11.9 by 12.3 micrometer and sporulated in 48 to 72 hr. Mouse-to-mouse tramsmission was achieved by injecting triturated tissue containing cysts. This is the third species of Besonitia to be cyclically transmitted by cats. |
