Variant Cell Lines of Haplopappus gracilis with Disturbed Activities of Nitrate Reductase and Nitrite Reductase |
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Authors: | Gilissen L J Barneix A J van Staveren M Breteler H |
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Affiliation: | Research Institute ITAL, P. O. Box 48, 6700 AA Wageningen, The Netherlands. |
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Abstract: | Selected variant cell lines of Haplopappus gracilis (Nutt) Gray that showed disturbed growth after transfer from an alanine medium to NO3− medium were characterized. The in vivo NO3− reductase activity (NRA) was lower in these lines than in the wild type. In vitro NRA assays suggest that decreased in vivo NRA was not caused by a lower amount of active enzyme. Cells of the variant lines revealed up to 75% lower extractable activity of NO2− reductase as compared with the wild type. This coincided with higher accumulation of NO2− by the variant than by the wild type cells after transfer from alanine medium to NO3− medium. NO2− accumulation was transient or continuous, depending on cell line, metabolic state of the cells, and light conditions. |
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