Using T-RFLP to Assess the Impact on Soil Microbial Communities by Transgenic Lines of Watermelon Rootstock Resistant to Cucumber Green Mottle Mosaic Virus (CGMMV) |
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Authors: | Hoonbok Yi Hyo-Jeong Kim Chang-Gi Kim Chee Hark Harn Hwan Mook Kim Sangkyu Park |
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Institution: | (1) Division of Environmental & Life Science, Seoul Women’s University, Seoul, 139-774, South Korea;(2) Bio-evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon-gun, 363-883, South Korea;(3) Biotechnology Institute, Nongwoo Bio Co., Yeoju, Gyeonggi-do, 469-885, South Korea;(4) Department of Biological Science, Ajou University, San #5, Woncheon-dong, Yeongtong-gu, Suwon, 443-749, South Korea; |
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Abstract: | To establish quantitative methods for ecological risk assessment, we assessed the impacts of transgenic watermelon rootstock
(Citrullus lanatus (Twinser) cv. Gongdae) that was resistant to cucumber green mottle mosaic virus. The diversity of soil bacteria and fungi
was monitored from May to July of 2005. Terminal restriction fragment length polymorphism (T-RFLP) was used with 16S ribosomal
RNA (rRNA) coding genes for bacterial communities and with internal transcribed spacer (ITS) regions of rRNA coding genes
for fungal communities. Multivariate analysis of variance (MANOVA) on the principal component analysis (PCA) scores of T-RF
profiles detected no significant difference between microbial communities with transgenic or non-transgenic watermelon. Likewise,
the results of our multi-response permutation procedure (MRPP) tests on non-metric multidimensional scaling (NMS) showed no
significant difference between plant types. However, both MANOVA on PCA and MRPP on NMS revealed significant changes in the
microbial community during the growing season. We used loading values of PCA to rank the abundances of bacterial species and
found increases of some species in June and July. |
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