Secondary structure-improved bioaffinity correlation in elongated and modified synthetic epitope peptides from p24 HIV-1 core protein |
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Authors: | Tonarelli Georgina Lottersberger Javier Salvetti Jorge L Jacchieri Saul Silva-Lucca Rosemeire A Beltramini Leila M |
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Institution: | 1.Departamento Química. Orgánica, Facultad de Bioquímica y Cs. Biológicas, UNL, Ciudad Universitaria, C.C. 242, 3000, Santa Fe, Argentina ;2.Funda??o Ant?nio Prudente, Centro de Pesquisas, Rua Prof Ant?nio Prudente 211, S?o Paulo, SP 01509-090, Brazil ;3.Departamento de Física e Informática, Grupo de Biofísica Molecular, Instituto de Física de S?o Carlos, S?o Paulo, Brazil ; |
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Abstract: | Summary In order to study the correlation between secondary structure and bioaffinity of long and modified sequences of p24 protein
from HIV-1, three peptides containing the minimal size epitope from region 208–217 (EAAEWDRVHP) were prepared. It was found
that peptide p24-1n, an elongated native sequence, has the lowest KD and a predominant α-helix structure in the presence of trifluoroethanol. Three peptides containing another epitope from region
293–302 (FRDYVDRFK) were also synthesized. We have observed that modifications on the native sequence p24-2n (region 287–308)
increased the α-helix content and this was correlated with an improvement of the recognition by antibodies in ELSA.
Abbreviations: CD, circular dichroism; DMF, N,N-dimethylformamide; DMSO, dimethyl sulfoxide; ELSA, enzyme linked immunosorbent assay; EDT,
1,2-ethanedithiol; Fmoc, fluorenylmethoxycarbonyl; FAB-MS, fast atom bombardment mass spectrometry; HPLC, high pressure liquid
chromatography; MeCN, acetonitrile; NMM, N-methylmorpholine; PyBOP, benzotriazole-1-yl-oxy-tris-pyrrolidino-phosphonium hexafluorophosphate;
SELCOM, self consistent method; TMB, 3,3′,5,5′-tetramethylbenzidine; TFA, trifluoroacetic acid; TFE, trifluoroethanol; Aminoacid
symbols denote the L-configurations. Abbreviations for amino acids and nomenclature of peptides follow the recommendations
of the IUPAC-IUB Commission on Biochemical Nomenclature (Eur. J. Biochem., 138 (1984) 9). |
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Keywords: | bioaffinity circular dichroism conformation immunoassays molecular modelling synthetic peptides |
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