Characterization of a proton translocating ATPase and sucrose uptake in a tonoplast-enriched vesicle fraction from sugarcane |
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Authors: | Lorraine Williams Margaret Thom rew Maretzki |
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Affiliation: | Dept of Biology, The Univ. of Southampton, Southampton SO9 5NH, UK;Hawaiian Sugar Planters' Association, 99–193 Aiea Heights Drive, Aiea, HI 96701, USA. |
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Abstract: | A sucrose gradient fraction was used to characterize the tonoplast ATPase from storage tissue of the sugarcane plant ( Saccharum sp. var. H57–5175). Marker enzyme analyses and characterization of low-density vesicles isolated on a sucrose gradient were consistent with a highly enriched tonoplast fraction. ATPase and proton transport activities were both substantially inhibited by nitrate (80%), but very little by vanadate (10%), indicating a high titer of tonoplast compared to plasma-membrane vesicles in the fraction. Sensitivity toward other inhibitors, as well as ion effects, correlated closely among ATPase and proton translocation activities. Although the vesicles in this fraction showed good proton translocating activity there was no indication that ATP stimulated sucrose uptake in this tonoplast population. |
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Keywords: | ATPase proton translocation Saccharum sucrose uptake sugarcane tonoplast vesicles |
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