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Abundance patterns of lily pollen cDNAs: characterization of three pollen-preferential cDNA clones
Authors:Seong-Ryong Kim  David Finkel  Yong-Yoon Chung  Gynheung An
Institution:(1) Present address: Department of Biology, Sogang University, Seoul, Republic of Korea;(2) Institute of Biological Chemistry, Washington State University, 99164-6340 Pullman, WA, USA;(3) Department of Genetics and Cell Biology, Washington State University, 99164 Pullman, WA, USA;(4) Department of Horticulture, Washington State University, 99164 Pullman, WA, USA
Abstract:Twenty-five clones were randomly selected from a mature pollen cDNA library of Easter lily (Lilium longiflorum Thunb.) in order to study the abundance of pollen-expressed mRNAs and the functional roles of the proteins encoded by these mRNAs. Plaque hybridization experiments were conducted to estimate indirectly the expression level of the mRNAs. Based on the hybridization frequency in the mature pollen library, the cDNA clones were divided into three abundance groups. Eight clones belonged to a high abundance class in which each cDNA clone was present in the mature lily pollen library at a frequency between 0.3 and 3%. Six of these clones were not found in cDNA libraries made from carpel, leaf, or root, suggesting that they are preferentially expressed in pollen. Fourteen clones belonged to a medium abundance class and were present in the mature pollen library at a frequency between 0.01 and 0.08%. The remaining three clones, which were present at a frequency below 0.01%, were grouped as a low abundance class. Almost all of the cDNA clones which belong to either the medium or low abundance class were also detected in the leaf library. Northern blot hybridization with three of the highly abundant cDNA clones confirmed their preferential expression in anther. In situ hybridization experiment with one of the clones showed the pollen-specific expression of the clone in mature anther. DNA sequence analysis revealed that the clone LMP131 encodes a peptide which is highly homologous to the tomato pollen-preferential gene, LAT59, which encodes a putative pectate lyase. The clone LMP134 encodes a peptide that shows an extensive similarity to a variety of thioredoxins. The third clone LMP132 encodes a 182-residue protein that has no significant homology to known sequences.
Keywords:Pollen  mRNA abundance  cDNA Pectate lyase  Thioredoxin
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