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Poliovirus and polio antibody assay in HEp-2 and Vero cell cultures |
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| Authors: | P Albrecht J C Enterline E J Boone M J Klutch |
| Affiliation: | 1. Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China;2. Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, China;3. Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China;4. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China |
| Abstract: | HEp-2 cell cultures were about three to 30 times more sensitive for poliovirus titration than Vero cells. Attenuated strains induced a complete cytopathic effect in HEp-2 but not in Vero cells. For polio antibody titration, HEp-2 and Vero cells were equally suitable. A high degree of sensitivity and reproducibility of virus neutralization was achieved in tests utilizing a low virus dose and serum-virus incubation overnight at 36 degrees C. Staining of infected trays with crystal violet obviated reading of viral CPE under the microscope and expedited the evaluation of larger-scale tests. |
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