| 光棘豆悬浮细胞原生质体培养影响因素的研究 |
| |
| 引用本文： | 张根发,周延清.光棘豆悬浮细胞原生质体培养影响因素的研究[J].植物研究,1999,19(3):313-317. |
| |
| 作者姓名： | 张根发 周延清 |
| |
| 作者单位： | [1]北京师范大学生物系 [2]河南师范大学生物系 |
| |
| 摘 要： | 采用光棘豆无菌苗胚轴诱导的分化能力强的愈伤组织建立的胚必细胞悬浮系材料，对悬浮细胞原生质体解离所需酶液，原生质体培养所需要的渗透压和激素组合进行了研究。发现较低的培养基渗透压（〈０．３５ｍｏｌ／Ｌ葡萄糖）和较高浓度的２，４－Ｄ（〉１ｍｇ／Ｌ）易于诱导细胞出芽分裂，导致细胞破碎和死亡，并引起培养细胞褐化，找出适合光棘悬浮细胞原生质培养基渗透压和激素组合。
|
| 关 键 词： | 光棘豆 原生质体 激素 渗透压 悬浮细胞 |
FACTORS AFFECTING PLANT REGENERATION FROM PROTOPLASTS DERIVED FROM CELL-SUSPENSION CULTURES OFOXYTROPIS LEPTOPHYLLA |
| |
| Zhang Gen-fa,Zhou Yan-qing,Zhang Fei-xiong,Li Xiao-yu.FACTORS AFFECTING PLANT REGENERATION FROM PROTOPLASTS DERIVED FROM CELL-SUSPENSION CULTURES OFOXYTROPIS LEPTOPHYLLA[J].Bulletin of Botanical Research,1999,19(3):313-317. |
| |
| Authors: | Zhang Gen-fa Zhou Yan-qing Zhang Fei-xiong Li Xiao-yu |
| |
| Institution: | Zhang Gen-fa 1 Zhou Yan-qing 2 Zhang Fei-xiong 3 Li Xiao-yu 2 |
| |
| Abstract: | Cell-suspension cultures derived from the calli initiated from plumalar axis of Oxytropis leptophylla were used for studying the effects of different enzymes, phytohormones and osmotic pressure in medium (e.g. the content of glucose) on protoplast isolation and culture. The results showed that the protoplasts were easy to damage into small segments when osmotic pressure was out of balance for protoplats' protoplasm. It was also found that the low osmotic pressure acted similarly to 2,4-D at high concentration in inducing the cell budding division, which resulted in death of cells. The optimum osmotic pressure and phytohormones combination was determined. |
| |
| Keywords: | Oxytropis leptophylla Protoplast Phytohormone Osmotic pressure Cell-suspension |
| 本文献已被 CNKI 维普 等数据库收录！ |
